Gene recombination in the conventional breeding is accomplished through sexual reproductive means including meiotic division and fertilization, while in the in vitro breeding it is carried out by non-reproductive genetic manipulation. In the former, tremendous amount of expenditure, manpower and facility is required, but in the latter, simple laboratory and a few reserchers are enough as this kind of breeding is carried out in the test tubes.
Present paper describes some of the tissue culture methods expected to play the pivotal roles in the future in vitro breeding, included being the production of synthetic seed, mutant selection from the haploid cell population, and the improvement of genetic characters of crop species through naked protoplasts.
|